Immobilization of Vigna Mungo β-amylase onto NaCl and NaNO Treated Woven Bombyx Mori Silk Fabrics

Vigna Mungo β-amylase was immobilized onto activated Bombyx mori silk fabric by enrichment with chlorination and treated with sodium nitrate (NaNO₃ ) via glutaraldehyde coupling. The immobilization of Vigna Mungo β-amylase onto activated Bombyx mori silk fabrics was excellent by  having 72-84% of retention of enzyme activity. The immobilization optimum conditions such as pH, reaction time, substrate concentration, CaCl₂ concentration and temperature were studied. Thermal stability of the enzyme was improved after immobilization which was 72°C as compared to free enzyme (40°C). The optimum substrate concentration and effect of CaCl₂ concentration was also carried out. In addition, the immobilized  enzymes had good storage stability and reusability by maintaining 60-70% of its activity after 90-120 days