In vitro Antioxidant and Anti-Inflammatory Activity of Dendrophthoe falcata Endophytic Fungal Ribosome Inactivating Proteins

Aim: The goal of the current investigation was to characterize endophytic fungal Ribosome Inactivating Protein (RIPs) and these RIPs evaluated to analyse potentiality of antioxidant and antiinflammatory property. Materials and Methods: Total five different endophytic fungi were subjected to identification of molecular weight by SDS-PAGE, PAS staining for glycoprotein identification and characterization of RIP by hemaglutination assay. Those RIPs evaluated for antioxidant (DPPH and FRAP) and anti-inflammatory activity (albumin denaturation study. anti-proteinase and antilipoxygenase assay). Results: 5 distinct types of endophytic fungus were isolated from the stem and leaves of Dendrophthoe falcata growing on Lagerstroemia speciosa. Obtained five endophytic fungal protein extracts exhibited the presence of glycoproteins with molecular weight 68 kDa. All endophytic fungal proteins are confirmed as glycoproteins in PAS staining, showed positive test for hemagglutination assay. The CcRIP and DpRIP show positive test for the erythrocytes of A, B and AB and all endophytes crude RIP also expressed the hemagglutination activity and it was depending on different blood groups. The CcRIP expressed the highest antioxidant activity in DPPH method followed by DpRIP, DpsRIP, CsRIP and DpRIP. In the FRAP assay, CcRIP and DpRIP protein extract exhibited significant antioxidant property. The CcRIP shows significant anti-inflammatory activity in all the three different methods (albumin denaturation study, anti-proteinase and anti-lipoxygenase assay). Conclusion: The study demonstrates that endophytic fungal RIPs are the potential sources of novel antioxidant and anti-inflammatory products.