Introduction: According to Ayurveda, there are ten-fold examination of patients, sarata (status of tissue excellence) is one of them. Acharya charak has mentioned total eight types of sarata which are used to assess the bala of an individual. Currently, no tools are available which is valid and reliable. Objectives: The primary objective was to develop a validated Dhatu Sarata Assessment Questionnaire (DSAQ) that will be easier to administer, less timeconsuming and more sensitive to even small changes. Materials and Methods: A questionnaire was prepared based on features explained in Charak Samhita. The study was conducted in the age group of 18-30 years among 250 healthy volunteers. Analysis of the questionnaire was evaluated based on validity and reliability test. Results: The content validity of the tool achieved satisfactory results. The reliability (inter-rater, intra-rater and internal consistency) and validity of the newly developed tool were found satisfactory on statistical analysis. Conclusion: The questionnaire has attained satisfactory level of validity and reliability test therefore this tool can be used for both research purposes and academics. This tool can be used for assessment of dhatu sara either by self-assessment or by an expert.
