The global problem of antibiotic resistance and the lack of novel antibiotics in the markets is soon to be a health care crisis. Silver nanoparticles are recently considered as a tool of the medical field, especially in therapeutic applications. Streptomyces sp. is a natural antibiotic producer and could be utilized for the production of nanoparticles enhanced antibiotics. In the present investigation, 17 actinomycetes were isolated from soil samples collected from various regions in Dawadmi governorate, Riyadh province. Isolate DW102 was identified as potent isolate based on the ability to synthesize silver nanoparticles along with broad spectrum antibacterial activity when compared with other isolates. The potent isolate DW102 was identified as Streptomyces sp. Biosynthesized AgNPs were characterized by UV-Vis Spectroscopy, Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDX). Bioynthesized Ag-NPs showed antimicrobial activity against Multi Drug Resistant (MDR) strains of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Klebsiella pneumonia. The combination of antibiotics with AgNPs showed an overall percentage increase in average fold-area of zone of inhibition (ZOI) in this study.