High Yield Production of Industrially Important Multi Enzymes by Aspergillus Foetidus using Solid State Fermentation

Asian Journal of Biological and Life Science,2016,5,1,71-77.
Published:April 2016
Type:Research Article
Author(s) affiliations:

Ashwini S1, 2 and Raghavendra M.P1,*

1Postgraduate Department of Microbiology, Maharani’s Science College for Women, JLB Road, Mysore 570 005, Karnataka, INDIA.

2Department of Microbiology, Bharathiyar University, Coimbatore, Tamil Nadu, INDIA.


Isolation and screening of Aspergillus foetidus strains for the biosynthesis of high yield multi enzymes such as amylase, protease, xylanase and pectinase was conducted using solid state fermentation wherein agrowaste rice and wheat bran was used as a fermentation substrate. Different fermentation parameters were evaluated for maximum enzymes production. The results revealed that highly significant enzyme secretion was observed in rice bran substrate with optimum temperature 28°C, pH 4-6, 40% moisture content, 0.2ml inoculum size (24 million spores/gram) and 48 hrs. of incubation period. Under these fermentation parameters maximum enzyme activity for amylase (9,145 U/g), protease (2,013 U/g), xylanase (14,641U/g) and pectinase (390 U/g) was achieved. The present study is successful in isolating a high yield multi enzyme producing A. foetidus strain and standardizing SSF parameters, which can be exploited industrially to achieve maximum enzyme yield.