Isolation and Identification of Thermotolerent Acetic Acid Bacteria from Waste Fruits

Asian Journal of Biological and Life Sciences,2020,9,2,209-213.
Published:September 2020
Type:Research Article
Author(s) affiliations:

Bikash Kumar Sahoo, Rashmi Ranjan Mishra, Bikash Chandra Behera*

Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar, Odisha, INDIA.


In the present study, thirteen thermotolerant acetic acid bacteria were isolated from waste pomegranate and apple fruit in Carr agar medium following the dilution plate technique method at 37°C. The bacterial strain capable to change the colour of the medium from green to yellow were considered as Acetobacter sp. Most of the bacterial isolate could able to tolerate 3-4% of ethanol concentration, change the colour and pH of the medium and produce acetic acid in the range of 1.1-15.2 g/L. Among thirteen bacterial strains, the bacterial strain PAAB-3 could able to tolerate maximum ethanol (5%) and produce maximum acetic acid (15.2g/L) at 37°C. On the basis of morphological and biochemical characteristics the bacterial isolate, PAAB-3 is tentatively identified as Acetobacter aceti.