Silver Nanoparticles Induced effect on in-vitro Callus Production in Bacopa monnieri

Priya Kalsaitkar; Jay Tanna; Anand Kumbhare; Sushma Akre; Chetan Warade; Nilesh Gandhare

Asian Journal of Biological and Life Sciences ,2014,3,3,167-172.

Published:December 2014

Type:Research Article

Authors:

Author(s) affiliations:

Priya Kalsaitkar¹, Jay Tanna², Anand Kumbhare¹, Sushma Akre¹, Chetan Warade³, Nilesh Gandhare⁴

¹Post Graduate Teaching Department of Biotechnology, Dr. Ambedkar College Nagpur, Maharashtra 440010, INDIA.

²Post graduate teaching Department of Chemistry, Rashtrasant Tukadoji Maharaj Nagpur University Nagpur, Maharashtra-440033, INDIA.

³Post Graduate Teaching Department of Biology, Rashtrasant Tukadoji Maharaj Nagpur University Nagpur, Maharashtra-440033, INDIA.

⁴Department of Chemistry, Nabira Science College, Katol, Maharashtra-441302, INDIA.

Abstract:

Bacopa monnieri has been chosen as model medicinal plant to study the induced effect of Silver nanoparticles on in-vitro callus production. Distinct morphological changes on callogenesis were observed on stress application. The effect of Silver nanoparticles on callus induction and autoclaving were studied within MS media and without MS media. The MS medium with the lower concentration (16X10³) of Silver nanoparticles capable for medium growth of callus as that control and simultaneously reducing the rate of contamination. Biosynthesis of Silver nanoparticles were carried out by using Fusarium oxysporium fungus and characterized by UV-Vis spectrophotometer and LM 20.

Keywords:Bacopa monnieri, Callus, LM 20, Silver nanoparticles