In India, Leucas aspera is commonly utilized as an ethnomedicinal herb to treat numerous ailments. The current investigations assessed the anti-inflammatory, antioxidant, anticancer, apoptotic, and antivenom properties of an aqueous extract of Leucas aspera. Some in vitro models were used to estimate antioxidant activity, such as the DPPH photometric assay, OH radical scavenging capacity, anti-inflammatory activity, Cyclooxygenase (COX), and Lipoxygenase (LOX), anticancer, apoptotic, and antivenom activity. The study found that plant extracts' antioxidant and anti-inflammatory effects might be attributed to inhibiting DPPH and OH, COX, and LOX enzymes, respectively, supporting the traditional usage of the aforementioned plants in inflammatory illnesses. The MTT experiment showed that the aqueous extract of Leucas aspera was highly selective (p<0.005) against HepG2 cells, causing morphological alterations. Based on the current data, it was determined that the aqueous extract of Leucas aspera exhibits a more potent and safer anti-inflammatory, antioxidant capabilities, anticancer and anti-venom agent because of its significant PLA2 inhibition.
