The present study deals with the isolation of Rhizobium bacteria from root nodules of Mimosa pudica, and to check its efficiency as bio-fertilizer in improving plant growth. Rhizobium sps SN01 was isolated from root nodules of Mimosa pudica and confirmed to be Rhizobium bacteria through following biochemical tests of indole, methyl red, Voges-Proskauer and citrate utilization (IMViC), urease, hydrogen sulphide, carbohydrate fermentation tests. Rhizobium sps SN01 was analysed for antibiotic sensitivity test towards three different antibiotics such as Azithromycin, Amoxicillin and Cephalosporin. To check the efficiency, seeds of legume crops like cow peas, peas, and fenugreek were treated with Rhizobium sps, SN01, sowed in soil and coco-peat to study its germination rate and effectiveness in plant growth resulting in root length, shoot length, protein content and chlorophyll content. Impact of Rhizobium sps SN01 bacteria in soil and coco-peat quality and improvement was also focused by analyzing micro and macro nutrients responsible for its quality to improve plant growth.