GC-MS Profiling and in vitro Assessment of Antioxidant and Neuroprotective Properties of Ethanolic and Acetone Extracts of Oldenlandia corymbosa L.

Asian Journal of Biological and Life Sciences,2022,11,1,188-192.
Published:May 2022
Type:Research Article
Authors:
Author(s) affiliations:

Babitha Mandapathil Chellappan1, Gomathi Venkatachalam2,*, Chakala Umamaheswara Reddy3, Kumar M4, Venketeswarawarlu BS1

1Vinayaka Mission’s College of Pharmacy, Vinayaka Mission Research Foundation (Deemed to be University), Ariyannur, Salem, Tamil Nadu, INDIA.

2Department of Pharmacology, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission Research Foundation (Deemed to be University), Ariyannur, Salem, Tamil Nadu, INDIA.

3Deputy Registrar (Invigilation), Sri Ramachandra institute of Higher Education and Research (D.U), Porur, Chennai, Tamil Nadu, INDIA.

4Department of Pharmaceutical Chemistry, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission Research Foundation (Deemed to be university), Ariyannur, Salem, Tamil Nadu, INDIA.

Abstract:

To investigate antioxidant and neuroprotective activity of ethanolic (OCWE) and acetone extract (OCWA) of Oldenlandia corymbosa plant and identification of phytochemical constituents present in both extracts by GC-MS analysis. The antioxidant potential of O. corymbosa was evaluated by DPPH and ABTS assays. MTT assay was used to test the plant’s neuroprotective effect on neuroblastoma cell lines. Phytoconstituents of said extracts have been quantified by GC-MS method. Ethanolic extract of plant showed lowest IC50 value (130.56μg/mL) compared to acetone extract (189.33μg/mL). More neuroprotective effect also exhibited by ethanolic extract. GC-MS analysis revealed the presence of numerous biologically active phytoconstituents in both extracts. On the basis of antioxidant and in-vitro neurotoxicity studies it is revealed that ethanolic extract of the plant showed more antioxidant and neuroprotective activity compared to the acetone extract and this may be due to the presence of antioxidant and anti-inflammatory fractions such as neophytadiene, squalene, stigmasterol and thunbergol.