Microbiological Studies on Clostridium perfringens Isolated from Commercial Poultry of Balochistan

Necrotic enteritis is one of the most common enteric disease in poultry and a major problem in terms of morbidity and financial cost worldwide. This study was conducted to determine the prevalence of Clostridium perfringens in Division of Balochistan, Pakistan.1000 samples were collected from commercial poultry; 68% samples were positive while 32% found negative for Clostridium perfringens. Area wise results showed that commercial poultry of Quetta division (19%) was most affected as compare to other divisions of Balochistan, 43.2% of broiler and 24.8% of layer were affected from necrotic enteritis, confirmed through gram staining, biochemical tests and Polymerase Chain Reaction. Polymerase Chain Reaction showed clear bands of 541bp of CPE gene. Antibiograms result showed that Clostridium perfringens was sensitive against Amoxycillin and Chloramphenicol while resistance to Sulfamethoxazole, Trimethoprim and Kanamycin. It was concluded that necrotic enteritis was caused by Clostridium perfringens that can be eradicated by treating with antibiotic Penicillin.