Field Evaluation and Serological Detection of Potato Leaf Roll Virus in Potato Germplasm

Waqas Ashraf; Amer Habib; Muhammad Ahmad Zeshan; Amina Amin; Safdar Ali; Muhammad Mohsin; Atta ur Rehman Khan; Muhammad Usman Ghani; Awais Ahmed Khan; Syed Wasim Hassan

Asian Journal of Biological and Life Sciences ,2020,9,1,15-19.

DOI:10.5530/ajbls.2020.9.3

Published:May 2020

Type:Research Article

Authors:

Author(s) affiliations:

Waqas Ashraf¹, Amer Habib², Muhammad Ahmad Zeshan^3,*, Amina Amin^4,7, Safdar Ali², Muhammad Mohsin², Atta ur Rehman Khan³, Muhammad Usman Ghani⁶, Awais Ahmed Khan³, Syed Wasim Hassan⁵

¹Department of Plant Pathology, Faculty of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, PAKISTAN.

²Department of Plant Pathology, University of Agriculture, Faisalabad, PAKISTAN.

³Department of Plant Pathology, College of Agriculture, University of Sargodha, PAKISTAN.

⁴Department of Mathematics, National College of Business Administration and Economics, Gulberg-III, Lahore, PAKISTAN.

⁵Department of Plant Breeding and Genetics, College of Agriculture, University of Sargodha, PAKISTAN.

⁶Institute of Soil and Environmental Sciences, University of Agriculture Faisalabad, PAKISTAN.

⁷Department of Mathematics, School of Science and Engineering, LUMS, Lahore Cantt. PAKISTAN.

Abstract:

Potato tuber (Solanum tuberosum L.), a main staple food ranked 4^th in the world followed by rice, wheat and maize. Potato leaf roll virus (PLRV) is the most vital pathogen of potato in Pakistan that cause 90% losses and transmitted by green peach aphid (Myzus persicae).Twenty cultivars of potato were screened against PLRV. Twenty samples were collected on the basis of symptoms and tested by serological assay (DAS-ELISA). Disease incidence was calculated using prescribed formulae. Among all tested cultivars, ten cultivars were found resistant, five cultivars moderately resistant, one cultivar moderately susceptible and two cultivars highly susceptible. Detection of PLRV was done by DAS-ELISA which indicated that three cultivars found resistant, eleven moderately resistant, two moderately susceptible and four cultivars were susceptible.

Keywords:Cultivars, ELISA, Management, PLRV, Resistance, Screening