Development of a Recombinant S1 Domain of the Porcine Epidemic Diarrhea Virus Spike Protein Based ELISA for Virus Detection

Asian Journal of Biological and Life Sciences,2016,5,2,125-130.
Published:August 2016
Type:Research Article
Author(s) affiliations:

Nguyen Van Noi 1,2,*, Yao-Chi Chung3, Ta-Chih Cheng1

1Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, TAIWAN.

2Institute of Life Science, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen, VIETNAM.

3Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, Pingtung, TAIWAN.


A recombinant S1 domain of the porcine epidemic diarrhea virus spike protein (S1), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The S1 gene of PEDV was expressed in Escherichia coli. The purified recombinant S1 protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the anti-PEDV-S1 antibody reacted with PEDV-infected cells. The antibody was utilized to develop an indirect ELISA to detect PEDV. Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive.