Production of Multiplex PCR Kit for Fraud Detection in Dairy Products

Asian Journal of Biological and Life Sciences ,2015,4,1,54-58.
Published:Aprill 2015
Type:Research Article
Author(s) affiliations:

Marjan Anvarhosseini, Ali Mohamadi Sani*

Department of Food science and Technology, Quchan Branch, Islamic Azad University, Quchan, IRAN.


In recent years, along with the growing purchasing power of the population, there has been an increasing demand for high quality product. The object of this study was the identification fraud and animal species (cattle, ovine and caprine) in the dairy products by using Multiplex PCR method. 30 different samples (milk, yogurt and cheeses) were collected from companies and local product. DNA extraction was done based on the guanidinium thiocianate-silicagel method. PCR tests were specific for each species and allowed detection in feedstuffs of levels as low as 1% cattle, ovine and caprine DNA. The length of the amplified fragments with bovine primer is 470 bp, ovine 584 bp and caprine 330 bp which are equal to the expected range. The results showed that 100% of the ovine and caprine cheeses were adulterated with bovine's milk. 50% of ovine milk samples and 60% of ovine yoghurt samples were contaminated with bovine's milk. Also, the high sensitivity of Multiplex PCR in identification of animal species and detection of dairy products fraud was shown.