Silver Nanoparticles Induced effect on in-vitro Callus Production in Bacopa monnieri

Asian Journal of Biological and Life Sciences ,2014,3,3,167-172.
Published:December 2014
Type:Research Article
Authors:
Author(s) affiliations:

Priya Kalsaitkar1, Jay Tanna2,  Anand Kumbhare1, Sushma Akre1,  Chetan Warade3,  Nilesh Gandhare4

1Post Graduate Teaching Department of Biotechnology, Dr. Ambedkar College Nagpur, Maharashtra 440010, INDIA.

2Post graduate teaching Department of Chemistry, Rashtrasant Tukadoji Maharaj Nagpur University Nagpur, Maharashtra-440033, INDIA.

3Post Graduate Teaching Department of Biology, Rashtrasant Tukadoji Maharaj Nagpur University Nagpur, Maharashtra-440033, INDIA.

4Department of Chemistry, Nabira Science College, Katol, Maharashtra-441302, INDIA.

Abstract:

Bacopa monnieri has been chosen as model medicinal plant to study the induced effect of Silver nanoparticles on in-vitro callus production. Distinct morphological changes on callogenesis were observed on stress application. The effect of Silver nanoparticles on callus induction and autoclaving  were studied within MS media and without MS media. The MS medium with the lower concentration (16X103) of Silver nanoparticles capable for medium growth of callus as that control and simultaneously reducing the rate of contamination. Biosynthesis of Silver nanoparticles were carried out by using Fusarium oxysporium fungus and characterized by UV-Vis spectrophotometer and LM 20.