Production and Scrutiny of Silver Nanoparticles From Corynebacterium glutamicum ATCC13032 and antimicrobial sensitivity assessment

Asian Journal of Biological and Life Sciences ,2014,3,2,82-89.
Published:August 2014
Type:Original Research
Author(s) affiliations:

B.Gowramma1 , G.S.Avadhani2 , M.Rafi1 , K.V.Chamundeswaramma 3*, D.Muralidhara Rao1

1Department of Biotechnology, Sri Krishna Devaraya University, Anantapuram -515003, Andhra Pradesh, INDIA.

2Department of Materials Engineering, Indian Institute of Sciences, Bangalore, Karnataka, INDIA.

3Department of Zoology, Government degree college (W), Khammam, Andhra Pradesh, INDIA.


In the present study, silver nanoparticles (AgNPs) were synthesized from C. glutamicum and their antibacterial activity has been investigated. Dried biomass of C. glutamicum was challenged with aqueous Diamine silver ([Ag (NH3 )2 ] ) containing 1mM AgNo3 at different pHconditions.Transmission electron microscopy (TEM) and Scanning electron microscope (SEM) analysis of the AgNPs were carried out. X- ray diffraction (XRD) results have shown that these nanoparticles exhibit a face-centered cubic crystal structure. Energy-dispersive X-ray (EDX) spectroscopy analysis for the confirmation of elemental silver was carried out for the detection of elemental silver. Fourier transform infrared (FTIR) spectra analysis shows that some functional groups, such as the carboxyl and amide of the proteins, were involved in the processes of bioreduction. Antimicrobial activity of the synthesized AgNPs against multi drug resistant bacteria and fungi was assessed by well diffusion method. AgNPs from C. glutamicum exhibited a potential antibacterial activity against the bacterial strains. The antimicrobial activity of silver nanoparticles was extremely good towards bacterial strains than the fungal strains. Thus, this study proved that the C. glutamicum ATCC13032 can produce AgNPs and the nanoparticles can be used as an effective antimicrobial substance.