Prosopis spicigera Sm. (Leguminasae) is a commonly used plant in Indian traditional medicine. In the current study anti-inflammatory activity of ethanolic extract of P. spicigera Seeds was investigated using different animal models. The extract was also subjected to phyto-chemical analysis and their toxic potential. The anti-inflammatory activity was measured using the carrageenan-induced paw edema test. The air dried seeds of P.spicigera were soaked in ethanol (1:4, w/v) at the temperature of 60oC for 72 h. The supernatant collected was evaporated under reduced pressure and kept at 5oC until used. The extract was emulsified using 0.1% Tween -80 in normal Saline to prepare the doses of 33, 100 and 300 mg kg -1 , before performing each experiment. Anti-inflammatory activity in a dose-dependant manner in all assays used to the extract administered intraperitoneally exhibited significant (p<0.05).