Anti-Inflammatory Activity of Seeds of Prosopis spicigera in Animals

Asian Journal of Biological and Life Science,2013,2,2,119-121.
Published:August 2013
Type:Research Article
Author(s) affiliations:

Rajiv Agrawal1,*, H. K. Garg3, Udita Garg2, S.K.Singh1

1Dept. of Chemistry, D.B.S. (P.G.) College, Kanpur-208006 Uttar Pradesh, INDIA.

2Dept. of Micro-biology, D.B.S. (P.G.) College, Kanpur-06 Uttar Pradesh, INDIA.

3Dept. of Chemistry, D.V. (P.G.) College, Orai-285001 Uttar Pradesh, INDIA.


Prosopis spicigera Sm. (Leguminasae) is a commonly used plant in Indian traditional medicine. In the current study anti-inflammatory activity of ethanolic extract of P. spicigera Seeds was investigated using different animal models. The extract was also subjected to phyto-chemical analysis and their toxic potential. The anti-inflammatory activity was measured using the carrageenan-induced paw edema test. The air dried seeds of P.spicigera  were soaked in ethanol (1:4, w/v) at the temperature of 60oC for 72 h. The supernatant collected was evaporated under reduced pressure and kept at 5oC  until used. The extract was emulsified using 0.1% Tween -80 in normal Saline to prepare the doses of 33, 100 and 300 mg kg -1 , before performing each experiment. Anti-inflammatory activity in a dose-dependant manner in all assays used to the extract administered intraperitoneally exhibited significant (p<0.05).