Aloe vera and vitamin E (natural antioxidants) are traditional medicine used to treat various disorders. The purpose of the study was to evaluate the hematological profile of herbal drugs. Drugs were showing the significant increase in hemoglobin concentration and erythrocyte count. In addition, Aloe vera and vitamin E (alpha-tocopherol) reduced the platelets count at long-term administration and increase in the bleeding time. Herbal drugs take part in the prevention of free radical formation and reduced the production of reactive oxygen specie by NADPH Oxidase and then prevent the rupture of erythrocytes by free radicals, thus improve the integrity of erythrocyte by decreasing the fragility of erythrocytes and maintain erythrocyte count and hemoglobin level. It suggested that pretreatment of Aloe vera and vitamin E have protective role in anemia against oxidative stress and have free radical scavenging activity after long- term administration.