In the present study, thirteen thermotolerant acetic acid bacteria were isolated from waste pomegranate and apple fruit in Carr agar medium following the dilution plate technique method at 37°C. The bacterial strain capable to change the colour of the medium from green to yellow were considered as Acetobacter sp. Most of the bacterial isolate could able to tolerate 3-4% of ethanol concentration, change the colour and pH of the medium and produce acetic acid in the range of 1.1-15.2 g/L. Among thirteen bacterial strains, the bacterial strain PAAB-3 could able to tolerate maximum ethanol (5%) and produce maximum acetic acid (15.2g/L) at 37°C. On the basis of morphological and biochemical characteristics the bacterial isolate, PAAB-3 is tentatively identified as Acetobacter aceti.