Phosphorus and nitrogen are very much crucial for growth of Plant. Nitrogen fixing bacteria and phosphate solubilizing bacteria (PSB) promote plant growth and enhance various cellular processes like root elongation, proliferation and changes of root architecture, seed development and normal crop maturity. In present study the inoculation effect of phosphate solubilizing and Nitrogen fixing bacteria, isolated from rhizosphere and non-rhizosphere soil of North Gujarat, India are investigated. The isolated bacteria were tested for indole acetic acid (IAA), ammonia production and phosphate solubilization in vitro. Various parameters like root and shoot height, number of root hair, length, width and weight of leaves and chlorophyll content under greenhouse condition was measured. The study shows highest IAA production 0.1822 μg mL-1 by isolate N8 and highest phosphate production 0.029 μg mL-1 by isolate P6. The DNA of effective bacterial isolates was amplified using 16S r-DNA primers. Amplified PCR product were purified and sequenced to identify the isolates up to species level using BLAST. The isolates were identified as Azotobacter vinelandii and Bacillus niabensis, respectively (Accession number: JX564632 and KF535156).