Field Evaluation and Serological Detection of Potato Leaf Roll Virus in Potato Germplasm

Asian Journal of Biological and Life Sciences ,2020,9,1,xx-xx.
DOI:
Published:May 2020
Type:Original Article
Authors:
Author(s) affiliations:

Waqas Ashraf1, Amer Habib2, Muhammad Ahmad Zeshan3,*, Amina Amin4,7, Safdar Ali2, Muhammad Mohsin2, Atta ur Rehman Khan3, Muhammad Usman Ghani6, Awais Ahmed Khan3, Syed Wasim Hassan5

1Department of Plant Pathology, Faculty of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, PAKISTAN.

2Department of Plant Pathology, University of Agriculture, Faisalabad, PAKISTAN.

3Department of Plant Pathology, College of Agriculture, University of Sargodha, PAKISTAN.

4Department of Mathematics, National College of Business Administration and Economics, Gulberg-III, Lahore, PAKISTAN.

5Department of Plant Breeding and Genetics, College of Agriculture, University of Sargodha, PAKISTAN.

6Institute of Soil and Environmental Sciences, University of Agriculture Faisalabad, PAKISTAN.

7Department of Mathematics, School of Science and Engineering, LUMS, Lahore Cantt. PAKISTAN.

Abstract:

Potato tuber (Solanum tuberosum L.), a main staple food ranked 4th in the world followed by rice, wheat and maize. Potato leaf roll virus (PLRV) is the most vital pathogen of potato in Pakistan that cause 90% losses and transmitted by green peach aphid (Myzus persicae).Twenty cultivars of potato were screened against PLRV. Twenty samples were collected on the basis of symptoms and tested by serological assay (DAS-ELISA). Disease incidence was calculated using prescribed formulae. Among all tested cultivars, ten cultivars were found resistant, five cultivars moderately resistant, one cultivar moderately susceptible and two cultivars highly susceptible. Detection of PLRV was done by DAS-ELISA which indicated that three cultivars found resistant, eleven moderately resistant, two moderately susceptible and four cultivars were susceptible.