Introduction: Nature has been an attractive source of new therapeutic candidate compounds. The plants produce naturally occurring secondary metabolites which are being investigated for their anticancer and antioxidant activities. Tithonia diversifolia and Gliricidia sepium are commonly growing in Bukidnon, Philippines. These plants have long list of ethno-medicinal uses. Thus, there is a need to further investigate their phytochemical constituents as well as their biological activities. Objectives: The study focuses on the phytochemicals in the ethanolic leaf extracts of T. diversifolia and G. sepium collected from Bukidnon, Philippines. Moreover, cytotoxic and antioxidant properties of the extracts were also determined through Brine Shrimp Lethality Assay (BSLA) and Total Antioxidant Capacity, respectively. Materials and Methods: Qualitative phytochemical screening and determination of Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Antioxidant Capacity (TAC) and cytotoxicity of the ethanolic leaf extracts of T. diversifolia and G. sepium were conducted. Results and Discussion: Results of the study showed the presence of flavonoids, phenolics, tannins and terpenoids in the ethanolic leaf extracts of T. diversifolia and G. sepium. The TPC of T. diversifolia (15.20 mg GAE/g dry sample) was found higher than that of G. sepium (14.43 mg GAE/g dry sample). Similar trend was also observed in the TFC where T. diversifolia and G. sepium recorded 12.50 and 9.00 mg QE/g dry sample, respectively. T. diversifolia also gave higher TAC of 302.8 mg AAE/ g dry sample than G. sepium (200.2 mg AAE/g dry sample). For cytotoxicity test, T. diversifolia (LC50 = 14.57 mg/L) exhibited more potent toxicity as compared to G. sepium (LC50 = 15.85 mg/L). The results indicate that the detected phytochemicals may account for the exhibited biological activities of T. diversifolia and G. sepium. Conclusion: This study uncovers the promising antioxidative and cytotoxic property of G. sepium and T. diversifolia that warrants further investigation.