The Effects of Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis Venoms on Human Cancer Cells

Asian Journal of Biological and Life Science,2013,2,1,50-53.
Published:April 2013
Type:Research Article
Author(s) affiliations:

Suchitra Khunsap1,*, Supranee Buranapraditkun2, Sunutcha Suntrarachun1, Songchan Puthong3, Orawan Khow1, Pannipa Chulasugandha1, Supatsorn Boonchang1

1Research of Development, Queen Saovabha Memorial Institute, Bangkok 10330, THAILAND.

2Cellular Immunology Laboratory Allergy and Clinical Immunology Unit, Department of Medicine, Faculty of Medicine Chulalongkorn, University, Bangkok 10330, THAILAND.

3Institute of Biotechnology and Genetic Engineering, Chulalongkorn University Institute Building 3, Phayathai Road, Patumwan, Bangkok 10330, THAILAND.


Three snake venoms: Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis were studied for cytotoxic activity and apoptosis on five cancer cell lines; BT474, SW620, KATO-III, Hep-G and ChaGo. Cytotoxic effect was determined by MTT assay and flow cytometry has 2 been used for apoptosis. Various concentrations of three venoms showed cytotoxic against cancer cells as time-dependent. The potent of cytotoxic from C. albolabris venom against cancer cells were: BT474 (2.96 ± 0.44 µg/ml), SW620 (3.32 ± 0.14 µg/ml), KATO-III (3.72 ± 0.11 µg/ml) and Hep-G (3.74 ± 0.43 µg/ml), respectively. C. rhodostoma venom showed cytotoxic to BT474 (3.16 ± 0.69 µg/ml), SW620 (3.5 ± 0.01 µg/ml) and KATO¬ 2 III (3.74 ± 0.37 µg/ml) while the venom of D. siamensis was highly toxic to only ChaGo (0.48 ± 0.04 µg/ml). Apoptotic cell death using PI staining was dose and time dependent.